Hoechst 33342 apoptosis
Nettet1. sep. 1994 · Abstract A flow cytometric method to detect apoptotic cells is described. This method is based on the detection of differences in chromatin condensation with … Nettet1. des. 2010 · Little is currently known about possible toxicity of fluorescent DNA dyes under CTLM conditions. In this study we demonstrate that the common live-cell DNA stain Hoechst 33342 can cause apoptosis under CTLM conditions. Although toxicity is evident at long times in the absence of imaging at high dye concentrations, phototoxicity from …
Hoechst 33342 apoptosis
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NettetThrough hematoxylin-eosin (HE) staining, Hoechst 33342 staining, transmission electron microscopy, and flow cytometry using FITC-Annexin V/PI double staining, 2,5-HD was … NettetHoechst 33342, a vital DNA-specific fluo-rochrome, was added for determinations of DNA content and subsequent cell cycle analysis (Fig. 1). There is a decrease in the …
Nettet1. sep. 2003 · Hoechst 33342 disrupts the 3 primary enzymatic reactions initiated in the minor groove of DNA, including DNA polymerase, 26, 30 RNA polymerase II, 13, 16, 21, 22, 31 and topoisomerase I. 16, 18–20, 22, 28 For example, Hoechst 33342–induced apoptosis decreases the TATA box-binding protein (TBP)/TATA box promoter complex … NettetInvitrogen Hoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used …
NettetHoechst 33342 is a specific fluorescent DNA stain that binds within the minor groove of double-stranded AT-rich regions. This stain can be used on both live and fixed cells (1). Portugal, J. and Waring, M.J. (1988) … NettetHoechst 33342 is a bis-benzimide derivative that binds to AT-rich sequences in the minor grove of double-stranded DNA. The emission is very bright, and for the purposes of the SP strategy, is detected at two emission wavelengths after UV excitation: Hoechst Red (630–650 nM) and Hoechst Blue (405–450 nM).
Nettet18. jun. 2016 · Furthermore, compound 1 dose-dependently induced A2780 cells apoptosis as evidenced by Hoechst 33342 staining, Annexin V positive cells, the up-regulated cleaved-PARP and the enhanced Bax/Bcl-2 ratio. What’s more, compound 1 also promoted the expression of the tumor suppressor p53 protein.
NettetHoechst 33342, 10 mg/mL in Water: 40046: 561.93: 10 mL: 350/461 nm: Yellow solution: Hoechst 33342, trihydrochloride trihydrate: 40047: 615.98: 100 mg: 350/461 nm: … normal gets you nowhereNettet29. apr. 2024 · Apoptosis is a physiological process that occurs commonly during the development of the preimplantation embryo. The present work examines the ability of apoptotic embryonic cells to express a... normal gdp and real gdpNettetHoechst 33258 nucleic acid stain is a popular nuclear counterstain that emits blue fluorescence when bound to dsDNA. Its uses are similar to Hoechst 33342 for counterstaining, apoptosis and cell cycle studies, but Hoechst 33258 is reportedly less cell-permeant. It also has been used to detect DNA in solution between 250ng/ml to … normal geographic area of workNettet25. des. 2008 · Hoechst 33342 is a kind of fluorescent dye used for evaluating the cell cycle and apoptosis in a variety of benign and malignant cell lines, such as BC3H-1 … normal gem scarlet and violetNettetThe Hoeschst 33342 is cell permeable and binds to dsDNA in live cells regardless of membrane status. Propidium iodide (PI) will permanently bind to DNA in dead cells. Together, you should be able... how to remove picc line with metal anchorNettetHoechst 33342 and 33258 are quenched by bromodeoxyuridine (BrdU), which is commonly used to detect dividing cells. Hoechst 33342 exhibits a 10 fold greater cell … how to remove picture background in coreldrawNettetHoechst 33,342 Staining. The fluorescent dye Hoechst 33,342 was used to visualize the nuclear fragmentation, the characteristic of apoptotic cell death. Typically, MCF-7 cells … how to remove pickle stain from shirt