Web1 jan. 2011 · One advantage of Hoechst 33342 is that it is membrane permeant and, thus, can stain live cells. Hoechst 33342 binds to adenine-thymine-rich regions of DNA in the minor groove. On binding to DNA, the fluorescence greatly increases. This protocol describes the use of Hoechst 33342 to label nuclear DNA of cells grown in culture. … WebLive cell imaging red nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342. Research Category Cell Imaging Research Sub Category Live Cell Dye Components 1) 1 vial of 50µL BioTracker 650 Red Nuclear Dye (1000X in DMSO) (CS224577)

Hoechst 33342 Solution - BD Biosciences

WebSince the Hoechst 33342 dye is specific for DNA binding, ribonuclease treatment is not needed to avoid nonspecific RNA staining. In addition to its use in fluorescence … WebObjective: To determine if Hoechst 33342 or Hoechst 33258 induces apoptosis in human promyelocytic leukemia cells (HL-60) and inhibits topoisomerase I activity. Design: A variety of methods were used to detect apoptosis: cell viability (trypan blue exclusion), nuclear fluorescence staining (Hoechst 33342 or Hoechst 33258 stained for 10 minutes ... resonance holdings llc

Analyzing Cell Death by Nuclear Staining with Hoechst 33342.

WebHoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish … WebOne of the first Hoechst derivatives developed, Hoechst 33258, was already used in 1969 for nuclear staining in living animals of cell culture and for karyotyping. In 1978, another DNA-binding fluorochrome, Hoechst 33342, was reported as a live stain of cells suitable for subsequent cell sorting. [ 5 ] WebHoechst 33342 is a popular cell-permeant, blue fluorescent nuclear stain. It is used to visualize the nuclei of living or fixed cells and tissues and is often used to distinguish condensed, pyknotic nuclei in apoptotic cells. Hoechst 33342 emits blue fluorescence when bound to double stranded DNA. It is slightly more membrane permeant than the ... resonance hitech city